6O38
Structure of a chaperone-substrate complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-22 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 |
| Unit cell lengths | 110.747, 118.489, 129.583 |
| Unit cell angles | 108.94, 103.97, 100.10 |
Refinement procedure
| Resolution | 35.788 - 2.595 |
| R-factor | 0.2255 |
| Rwork | 0.225 |
| R-free | 0.23990 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.465 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MLPHARE |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.640 |
| High resolution limit [Å] | 2.595 | 2.600 |
| Rpim | 0.040 | 0.590 |
| Number of reflections | 173168 | 8646 |
| <I/σ(I)> | 16.7 | 1.26 |
| Completeness [%] | 96.8 | 96 |
| Redundancy | 3.5 | 3.5 |
| CC(1/2) | 0.990 | 0.960 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 1.28 M magnesium sulfate, 0.1 M MES buffer, pH 6.5 |
