6O30
Lipid A transporter MsbA from Salmonella typhimurium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2013-02-17 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97945 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 82.257, 153.200, 232.483 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.754 - 4.470 |
R-factor | 0.3083 |
Rwork | 0.307 |
R-free | 0.33950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6bl6 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.779 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.800 | 4.810 |
High resolution limit [Å] | 4.470 | 4.470 |
Rmerge | 0.114 | 1.660 |
Rmeas | 0.138 | 2.010 |
Rpim | 0.077 | 1.110 |
Number of reflections | 15470 | 683 |
<I/σ(I)> | 10 | |
Completeness [%] | 85.0 | 75.8 |
Redundancy | 2.8 | 2.9 |
CC(1/2) | 0.950 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | MSBA PURIFIED IN DETERGENT MICELLES (UDM/FA231) IS MIXED 1: 1 WITH WELL SOLUTION (20 MM TRIS-HCL (PH 7.5) AND 27.5% PEG 300). |