6O2W

Crystal structure of the SARAF luminal domain Cys-lock mutant dimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-06-26
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.00
Spacegroup name	P 1
Unit cell lengths	24.910, 30.790, 76.140
Unit cell angles	88.58, 86.03, 89.79

Refinement procedure

Resolution	37.930 - 2.101
R-factor	0.1775
Rwork	0.175
R-free	0.23350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6o2u
RMSD bond length	0.007
RMSD bond angle	0.934
Data reduction software	MOSFLM
Data scaling software	Aimless (0.1.27)
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.930	2.160
High resolution limit [Å]	2.100	2.100
Rmerge	0.076	0.939
Rmeas	0.089	1.095
Rpim	0.046	0.561
Total number of observations	41899
Number of reflections	11188	461
<I/σ(I)>	12.3
Completeness [%]	85.1	43.9
Redundancy	3.7	3.8
CC(1/2)	0.997	0.609

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	3-5% glycerol or ethylene glycol