6O2V
Crystal structure of the SARAF luminal domain Cys-lock mutant monomer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-04-13 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.116 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.689, 60.953, 63.441 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.954 - 1.580 |
R-factor | 0.1674 |
Rwork | 0.165 |
R-free | 0.20320 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6o2u |
RMSD bond length | 0.006 |
RMSD bond angle | 0.854 |
Data reduction software | XDS |
Data scaling software | SCALA (3.3.20) |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 63.441 | 63.441 | 1.660 |
High resolution limit [Å] | 1.580 | 4.990 | 1.580 |
Rmerge | 0.031 | 0.519 | |
Rmeas | 0.099 | 0.036 | 0.651 |
Rpim | 0.038 | 0.014 | 0.305 |
Number of reflections | 32028 | 1154 | 4214 |
<I/σ(I)> | 16.2 | 17.3 | 1.5 |
Completeness [%] | 98.7 | 99.9 | 91.5 |
Redundancy | 6.6 | 6.4 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.4 | 293 | 0.1M sodium acetate, pH 4.2-4.6 and 1.2-1.6M sodium formate |