6O2U

Crystal structure of the SARAF luminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-10-06
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.127
Spacegroup name	C 1 2 1
Unit cell lengths	50.980, 63.930, 79.920
Unit cell angles	90.00, 100.68, 90.00

Refinement procedure

Resolution	39.432 - 1.800
R-factor	0.1951
Rwork	0.193
R-free	0.22750
Structure solution method	SIRAS
RMSD bond length	0.007
RMSD bond angle	0.934
Data reduction software	MOSFLM
Data scaling software	Aimless (0.2.17)
Phasing software	SHARP
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.432	1.840
High resolution limit [Å]	1.800	1.800
Rmerge	0.070	1.819
Rmeas	0.075	2.013
Rpim	0.028	0.846
Number of reflections	23024	1167
<I/σ(I)>	12.1
Completeness [%]	98.2	83.7
Redundancy	6.9	5.4
CC(1/2)	0.999	0.349

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.1M BisTris, pH 6.5, 18-20% PEG 5000 MME