6O2D

Schizosaccharomyces pombe Cnp3 Cupin Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	105
Detector technology	CCD
Collection date	2016-06-15
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.9786
Spacegroup name	P 41 21 2
Unit cell lengths	55.160, 55.160, 206.640
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.074 - 2.520
R-factor	0.2188
Rwork	0.217
R-free	0.25100
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.248
Data reduction software	XDS
Data scaling software	XDS
Phasing software	AutoSol
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.440	2.590
High resolution limit [Å]	2.520	2.520
Rpim	0.016	0.156
Number of reflections	11544	804
<I/σ(I)>	32.9
Completeness [%]	99.6
Redundancy	13.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298.15	Protein was mixed with 0.1 M HEPES (pH 7.0), 20% PEG 3350, and 6% w/v Trimethylamine N-oxide dihydrate in a 1:1 ratio (v/v). The reservoir solution was made up of 0.25 M potassium fluoride, 0.125 M HEPES pH 7, and 25% PEG 3350