6O2A
Crystal structure of 4493 Fab in complex with circumsporozoite protein NDN and anti-kappa VHH domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-12-14 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.033200 |
| Spacegroup name | P 1 |
| Unit cell lengths | 82.654, 90.728, 92.515 |
| Unit cell angles | 89.38, 64.29, 76.88 |
Refinement procedure
| Resolution | 29.415 - 2.150 |
| R-factor | 0.1932 |
| Rwork | 0.193 |
| R-free | 0.22110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | In house model |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.180 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.070 | 0.544 |
| Rpim | 0.044 | 0.339 |
| Number of reflections | 124730 | 5069 |
| <I/σ(I)> | 10.5 | 1.7 |
| Completeness [%] | 97.7 | 96.9 |
| Redundancy | 3.5 | 3.5 |
| CC(1/2) | 0.997 | 0.722 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 20 % (w/v) PEG3350, 0.2 M potassium nitrate |
