6O25
Crystal structure of 3945 Fab in complex with circumsporozoite protein NANP3 and anti-Kappa VHH domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-2 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-08-03 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.979360 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 156.249, 230.737, 48.249 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.733 - 2.900 |
R-factor | 0.218 |
Rwork | 0.215 |
R-free | 0.26840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Inhouse model |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.950 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.242 | 0.816 |
Rpim | 0.065 | 0.221 |
Number of reflections | 39925 | 1998 |
<I/σ(I)> | 10 | 1.7 |
Completeness [%] | 99.9 | 100 |
Redundancy | 14.8 | 14.7 |
CC(1/2) | 0.999 | 0.664 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.2 M di-sodium hydrogen phosphate, 20 % (w/v) PEG 3350 |