6O24
Crystal structure of 4498 Fab in complex with circumsporozoite protein NANP3 and anti-Kappa VHH domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-2 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-07-03 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.979341 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 62.998, 76.785, 63.657 |
Unit cell angles | 90.00, 98.94, 90.00 |
Refinement procedure
Resolution | 19.968 - 1.400 |
R-factor | 0.1616 |
Rwork | 0.161 |
R-free | 0.18620 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Internal Fab model |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.420 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.078 | 0.625 |
Rpim | 0.033 | 0.260 |
Number of reflections | 117560 | 4896 |
<I/σ(I)> | 12.7 | 1.6 |
Completeness [%] | 99.9 | 100 |
Redundancy | 6.7 | 6.8 |
CC(1/2) | 0.998 | 0.791 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 2.5 | 298 | 10 % (w/v) 2-methyl-2,4-pentanediol, 0.1 M citric acid pH 2.5 |