6O1M
Architectural principles for Hfq/Crc-mediated regulation of gene expression. Hfq-Crc-amiE 2:4:2 complex
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | FEI FALCON III (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.15 |
| Number of Particle | 435000 |
| Software | RELION,PHENIX,REFMAC,EPU,Gctf,UCSF Chimera,MOLREP |
| Symmetry | C2 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
