6O1E

The crystal structure of human MORC3 ATPase-CW in complex with AMPPNP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2018-06-03
Detector	RDI CMOS_8M
Wavelength(s)	1.0
Spacegroup name	P 31 2 1
Unit cell lengths	119.510, 119.510, 76.880
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.930 - 2.410
R-factor	0.1814
Rwork	0.179
R-free	0.23180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ix1
RMSD bond length	0.009
RMSD bond angle	1.036
Data reduction software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.930	2.500
High resolution limit [Å]	2.410	2.410
Number of reflections	24772
<I/σ(I)>	50.4
Completeness [%]	99.9
Redundancy	43.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	296	0.1 M HEPES, 10 % w/v PEG8000 and 8 % v/v ethylene glycol