6O07
Structure and mechanism of acetylation by the N-terminal dual enzyme NatA/Naa50 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2018-11-02 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 84.437, 125.726, 145.924 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.658 - 2.702 |
| R-factor | 0.2235 |
| Rwork | 0.222 |
| R-free | 0.25030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xnh |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.684 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.000 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.110 | 0.180 |
| Number of reflections | 43295 | 3314 |
| <I/σ(I)> | 13.11 | |
| Completeness [%] | 99.3 | 99.1 |
| Redundancy | 6.1 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 10% PEG3350, 0.1 M sodium malonate, pH 5.0 |
