6NZX
Hadesarchaea YNP_N21 cytochrome b5 domain protein (KUO41884.1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-05 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.95370 |
| Spacegroup name | P 63 |
| Unit cell lengths | 72.170, 72.170, 22.810 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.22266 |
| Rwork | 0.221 |
| R-free | 0.26067 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1j03 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 2.350 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.620 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.179 | 2.100 |
| Rmeas | 0.183 | 2.152 |
| Rpim | 0.040 | 0.470 |
| Number of reflections | 5554 | 363 |
| <I/σ(I)> | 15.8 | 1.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 20.7 | 20.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 296.15 | 0.1M sodium citrate, 2M ammonium sulfate, pH 5.0 |
