6NZI
Low resolution crystal structure of the bacterial multidrug efflux transporter AcrB in the presence of cadmium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-07-13 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.97857 |
Spacegroup name | H 3 2 |
Unit cell lengths | 144.280, 144.280, 519.520 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.797 - 4.440 |
R-factor | 0.2629 |
Rwork | 0.260 |
R-free | 0.31990 |
Data reduction software | XDS |
Data scaling software | xia2 |
Phasing software | MrBUMP |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.797 | 4.597 |
High resolution limit [Å] | 4.440 | 4.440 |
Number of reflections | 13142 | 1297 |
<I/σ(I)> | 11 | 3 |
Completeness [%] | 98.5 | 100 |
Redundancy | 8.9 | 9.3 |
CC(1/2) | 0.998 | 0.840 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | Tris, pH=7.5, 250 mM choline chloride, 10% PEG 2K MME, 5 mM CdSO4 |