6NZA
Crystal structure of E. coli fumarase C K324A variant with closed SS Loop at 1.41 angstrom resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-06-13 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.9787 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 103.232, 217.563, 86.383 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 59.342 - 1.406 |
R-factor | 0.1461 |
Rwork | 0.145 |
R-free | 0.17000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fuo |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 93.270 | 1.430 |
High resolution limit [Å] | 1.406 | 1.406 |
Rmerge | 0.122 | 0.118 |
Number of reflections | 187824 | 9292 |
<I/σ(I)> | 0.129 | 2.1 |
Completeness [%] | 100.0 | 100 |
Redundancy | 14.5 | 13.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.9 | 298 | PEG 3350, citrate |