6NYX

Human parainfluenza virus type 3 fusion protein N-terminal heptad repeat domain+VI

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2016-02-13
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97856
Spacegroup name	P 3
Unit cell lengths	88.030, 88.030, 75.750
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.120 - 1.850
R-factor	0.1988
Rwork	0.198
R-free	0.22880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ztm
RMSD bond length	0.018
RMSD bond angle	1.563
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.120	1.916
High resolution limit [Å]	1.850	1.850
Rmeas	0.077	1.254
Rpim	0.032	0.537
Number of reflections	56066	5640
<I/σ(I)>	12.85	1.35
Completeness [%]	99.8	99.96
Redundancy	5.7	5.4
CC(1/2)	0.999	0.660

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	30 mM NaF, 30 mM NaBr, 30 mM NaI, 20% (v/v) PEG 500 MME, 10% (w/v) PEG 20000 in 100 mM imidazole/MES monohydrate buffer (pH 6.5)