6NYX
Human parainfluenza virus type 3 fusion protein N-terminal heptad repeat domain+VI
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-02-13 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97856 |
Spacegroup name | P 3 |
Unit cell lengths | 88.030, 88.030, 75.750 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.120 - 1.850 |
R-factor | 0.1988 |
Rwork | 0.198 |
R-free | 0.22880 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ztm |
RMSD bond length | 0.018 |
RMSD bond angle | 1.563 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.120 | 1.916 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmeas | 0.077 | 1.254 |
Rpim | 0.032 | 0.537 |
Number of reflections | 56066 | 5640 |
<I/σ(I)> | 12.85 | 1.35 |
Completeness [%] | 99.8 | 99.96 |
Redundancy | 5.7 | 5.4 |
CC(1/2) | 0.999 | 0.660 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 30 mM NaF, 30 mM NaBr, 30 mM NaI, 20% (v/v) PEG 500 MME, 10% (w/v) PEG 20000 in 100 mM imidazole/MES monohydrate buffer (pH 6.5) |