Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | AREA DETECTOR |
| Collection date | 2004-06-06 |
| Detector | SBC-3 |
| Wavelength(s) | 0.97895 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 56.943, 56.943, 89.983 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.004 - 1.369 |
| R-factor | 0.1488 |
| Rwork | 0.148 |
| R-free | 0.16900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3KWT |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | PHENIX (1.14_3260: ???) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 30.004 | 1.390 |
| High resolution limit [Å] | 1.369 | 3.720 | 1.370 |
| Rmerge | 0.055 | 0.028 | 0.464 |
| Number of reflections | 31875 | 1784 | 1483 |
| <I/σ(I)> | 15.3 | ||
| Completeness [%] | 99.8 | 99.2 | 96.4 |
| Redundancy | 11.6 | 11.6 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 298 | 30% PEG-MME 2000, 0.1 M bis-tris propane pH 6.8, 0.1 M NaCl, 0.1 MM CaCl2, 0.5 mM TCEP |
