6NYP
Crystal structure of UL144/BTLA complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-01-31 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 2.0663 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 66.990, 77.200, 101.720 |
Unit cell angles | 90.00, 91.67, 90.00 |
Refinement procedure
Resolution | 39.930 - 2.700 |
R-factor | 0.232 |
Rwork | 0.230 |
R-free | 0.27650 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.355 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 39.930 | 39.930 | 2.770 |
High resolution limit [Å] | 2.700 | 12.080 | 2.700 |
Rmerge | 0.163 | 0.043 | 3.853 |
Rmeas | 0.164 | 0.044 | 3.889 |
Number of reflections | 28639 | 608 | 4085 |
<I/σ(I)> | 26.91 | 114.76 | 1.45 |
Completeness [%] | 99.5 | 97 | 97.9 |
Redundancy | 53.66 | 59.613 | 53.34 |
CC(1/2) | 1.000 | 1.000 | 0.615 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 297 | 0.1 M CHES pH 9.5, 20 % W/V PEG 8000 |