6NY9
Alpha/beta hydrolase domain-containing protein 10 from mouse
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-11-14 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.979 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 99.388, 99.388, 149.597 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 56.460 - 1.660 |
R-factor | 0.1963 |
Rwork | 0.195 |
R-free | 0.21880 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3llc |
RMSD bond length | 0.011 |
RMSD bond angle | 1.034 |
Phasing software | PHENIX (1.13-2998) |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 56.460 | 1.717 |
High resolution limit [Å] | 1.657 | 1.657 |
Rmerge | 0.108 | |
Number of reflections | 52270 | 4994 |
<I/σ(I)> | 20.82 | 1.6 |
Completeness [%] | 92.8 | 76.98 |
Redundancy | 12.9 | 12.8 |
CC(1/2) | 0.998 | 0.686 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIQUID DIFFUSION | 6.4 | 277.15 | 0.08 M strontium chloride hexahydrate, 0.04 M sodium cacodylate trihydrate pH 6.4, 25% v/v (+/-)-2-methyl-2,4-pentanediol (MPD), 0.012 M spermine tetrahydrochloride |