6NY6

Structure of dimeric Escherichia coli toxin YoeB bound to the Thermus thermophilus 30S ribosome

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-12-12
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	P 41 21 2
Unit cell lengths	401.710, 401.710, 175.440
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.826 - 3.740
R-factor	0.2067
Rwork	0.206
R-free	0.22420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1j5e
RMSD bond length	0.008
RMSD bond angle	1.163
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (dev_3406)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.826	3.874
High resolution limit [Å]	3.740	3.740
Rmerge	0.068	0.485
Number of reflections	145546	9392
<I/σ(I)>	10.95	1.08
Completeness [%]	96.9	85.1
Redundancy	3.29	2.17
CC(1/2)	0.999	0.673

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	MPD, AMMONIUM CHLORIDE, POTASSIUM CHLORIDE, MAGNESIUM ACETATE, SODIUM CACODYLATE, pH 6.5