6NXF

Crystal structure of mouse REC114 PH domain in complex with ANKRD31 C terminus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-09
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9791
Spacegroup name	P 43 21 2
Unit cell lengths	71.811, 71.811, 154.983
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.254 - 2.791
R-factor	0.2273
Rwork	0.222
R-free	0.27420
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.157
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.790	2.800
Rmerge	0.082	0.775
Number of reflections	10723	1045
<I/σ(I)>	23.3	1.9
Completeness [%]	99.9	99.6
Redundancy	7.5	6.3
CC(1/2)	0.981

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M Bis-Tris pH 5.5, 25% PEG 3350