6NX5

Crystal structure of the RRM domain of S. pombe Puf1 in the P21 space group

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2017-02-18
Detector	RAYONIX MX300-HS
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	46.854, 37.592, 73.930
Unit cell angles	90.00, 94.20, 90.00

Refinement procedure

Resolution	38.222 - 1.554
R-factor	0.195
Rwork	0.193
R-free	0.23130
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.580
High resolution limit [Å]	1.550	4.210	1.550
Rmerge	0.061	0.056	0.549
Rmeas	0.068	0.063	0.610
Rpim	0.029	0.029	0.260
Total number of observations	201031
Number of reflections	36421	1699	1655
<I/σ(I)>	13.2
Completeness [%]	98.1	87.1	90.9
Redundancy	5.5	5	5
CC(1/2)		0.991	0.843

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	15% w/v PEG3350, 0.1 M MES