6NWY

Modified tRNA(Pro) bound to Thermus thermophilus 70S (near-cognate)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-12
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	210.740, 450.260, 626.110
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.942 - 3.500
R-factor	0.2409
Rwork	0.240
R-free	0.25790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4v5c
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.14_3260)
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.942	49.942	3.290
High resolution limit [Å]	3.200	14.330	3.200
Rmerge	0.175	0.031	0.754
Rmeas	0.201	0.035	0.982
Total number of observations	3283538
Number of reflections	863724	10860	43415
<I/σ(I)>	6.77	32.11	1.02
Completeness [%]	89.8	94.5	61.4
Redundancy	3.802	4.641	1.857
CC(1/2)	0.995	0.999	0.327

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.6	293	0.1 M Tris-HOAc, pH 7.6, 0.15 M L-arginine HCl, 2.9% (v/v) PEG 20K, 12% (v/v) MPD, 0.0005M BME