6NWW

Crystal structure of the RRM domain of S. pombe Puf1 in the P212121 space group

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2017-01-03
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	54.692, 63.896, 79.544
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.490 - 2.060
R-factor	0.1776
Rwork	0.172
R-free	0.22510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6nx5
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.100
High resolution limit [Å]	2.060	5.590	2.060
Rmerge	0.080	0.054	0.206
Total number of observations	63326
Number of reflections	17513	888	838
<I/σ(I)>	9.2
Completeness [%]	97.9	88.4	95.1
Redundancy	3.6	3.8	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	3.6	293	0.3 M sodium chloride, 0.1 M citric acid, 20% glycerol