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6NWH

Structures of the transcriptional regulator BgaR, a lactose sensor.

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron siteAustralian Synchrotron
BeamlineMX2
Temperature [K]100
Detector technologyPIXEL
Collection date2018-09-26
DetectorDECTRIS EIGER X 16M
Wavelength(s)1.008020
Spacegroup nameP 2 21 21
Unit cell lengths46.168, 86.568, 119.812
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution46.210 - 2.030
R-factor0.18009
Rwork0.179
R-free0.20634
Structure solution methodSAD
RMSD bond length0.009
RMSD bond angle1.451
Data reduction softwareADDREF
Data scaling softwareAimless
Phasing softwareCRANK2
Refinement softwareREFMAC (5.8.0238)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]46.2002.080
High resolution limit [Å]2.0302.030
Rmerge0.1960.749
Rpim0.0400.230
Number of reflections316712137
<I/σ(I)>144.2
Completeness [%]99.492.5
Redundancy24.211.3
CC(1/2)0.9970.859
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP5.52935 mg/mL protein; 1.33 M sodium malonate, 100 mM citrate pH 5.5 in 200 nL plus 200 nL drops at 20 C

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