6NWE

Crystal structure of bovine opsin with beta octyl glucoside bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	173
Detector technology	PIXEL
Collection date	2016-10-22
Detector	DECTRIS EIGER R 4M
Wavelength(s)	1.033
Spacegroup name	H 3 2
Unit cell lengths	242.169, 242.169, 110.716
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.100 - 2.705
R-factor	0.1976
Rwork	0.196
R-free	0.22330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4j4q
RMSD bond length	0.005
RMSD bond angle	0.842
Data reduction software	XDS
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.100	2.714
High resolution limit [Å]	2.700	2.705
Rmerge		0.689
Rmeas		0.756
Rpim		0.308
Number of reflections	33699	2077
<I/σ(I)>	16.6
Completeness [%]	99.3	99.7
Redundancy	5.7	5.9
CC(1/2)		0.811

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	3.1-3.3M ammonium sulfate, 0.1M sodium acetate buffer, pH 5.5