6NVW
Crystal structure of penicillin G acylase from Bacillus megaterium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-12 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.0003 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.238, 77.961, 84.086 |
| Unit cell angles | 90.00, 101.67, 90.00 |
Refinement procedure
| Resolution | 46.032 - 2.203 |
| R-factor | 0.2175 |
| Rwork | 0.215 |
| R-free | 0.27470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_3386) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.032 | 2.380 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 25584 | |
| <I/σ(I)> | 9 | |
| Completeness [%] | 68.4 | |
| Redundancy | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 309 | 200 mM MgCl2, 20% (w/v) PEG 3350 |
