6NVP

Crystal structure of Pseudomonas putida nuclease MPE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-12
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.7712
Spacegroup name	P 61 2 2
Unit cell lengths	45.394, 45.394, 317.403
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.312 - 2.000
R-factor	0.1974
Rwork	0.192
R-free	0.24340
Structure solution method	SIRAS
RMSD bond length	0.007
RMSD bond angle	0.823
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXCD
Refinement software	PHENIX ((1.14_3211: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.070
High resolution limit [Å]	2.000	2.000
Rpim	0.031	0.345
Number of reflections	14173
<I/σ(I)>	28.1	2
Completeness [%]	98.3	91.5
Redundancy	4.3	2.4
CC(1/2)	0.995	0.794

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	293	0.1 mM Tris-HCl pH 7.4, 20% PEG MME 2000, 5mM MnCl2