6NVO
Crystal structure of Pseudomonas putida nuclease MPE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-12-12 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.7712 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 58.224, 72.995, 41.748 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.498 - 2.196 |
R-factor | 0.2079 |
Rwork | 0.202 |
R-free | 0.26310 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6nvp |
RMSD bond length | 0.009 |
RMSD bond angle | 1.060 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXCD |
Refinement software | PHENIX ((1.14_3211: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.240 |
High resolution limit [Å] | 2.196 | 2.196 |
Rpim | 0.034 | 0.194 |
Number of reflections | 8980 | |
<I/σ(I)> | 27.8 | 4.4 |
Completeness [%] | 93.9 | 72.3 |
Redundancy | 4.6 | 3.3 |
CC(1/2) | 0.925 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 293 | 0.1 mM Tris-HCl pH 7.2, 16% PEG MME 2000, 5mM MnCl2 |