6NVM

Crystal structure of 23S rRNA methyltransferase ErmE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-12
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.9677
Spacegroup name	P 43 21 2
Unit cell lengths	76.040, 76.040, 104.920
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.549 - 1.750
R-factor	0.1935
Rwork	0.192
R-free	0.21850
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.586
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.549	1.810
High resolution limit [Å]	1.750	1.750
Rmerge	0.093
Rmeas	0.096
Rpim	0.025
Number of reflections	31708	3116
<I/σ(I)>	19.04	0.77
Completeness [%]	99.9	99.84
Redundancy	15
CC(1/2)	1.000	0.324

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	294	2% (v/v) Tacsimate pH 5.0, 0.1 M Sodium citrate tribasic dihydrate pH 5.6 and 16% (w/v) PEG 3350