6NUO

Modified tRNA(Pro) bound to Thermus thermophilus 70S (cognate)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-27
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	210.170, 451.466, 620.210
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	152.459 - 3.200
R-factor	0.2257
Rwork	0.224
R-free	0.25610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4v5c
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	152.459	152.459	3.280
High resolution limit [Å]	3.200	14.310	3.200
Rmerge	0.255	0.047	0.717
Rmeas	0.276	0.050	0.834
Total number of observations	6265043
Number of reflections	951506	10854	69950
<I/σ(I)>	7.27	30.08	1.61
Completeness [%]	99.2	94.7	99
Redundancy	6.584	7.566	3.679
CC(1/2)	0.993	0.995	0.540

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.6	293	0.1 M Tris-HOAc, pH 7.6, 0.15 M L-arginine HCl, 2.9% (v/v) PEG 20K, 12% (v/v) MPD, 0.0005M BME