6NUM
The structure of GH32 from Bifidobacteium adolescentis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | BRUKER D8 QUEST |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-01-27 |
| Detector | APEX II CCD |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 88.630, 88.630, 114.570 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.378 - 1.900 |
| R-factor | 0.2367 |
| Rwork | 0.235 |
| R-free | 0.27020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.648 |
| Data reduction software | TRUNCATE |
| Data scaling software | SAINT |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14_3260)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.910 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.130 | 0.748 |
| Rmeas | 0.137 | 0.790 |
| Rpim | 0.043 | 0.252 |
| Number of reflections | 41542 | 2636 |
| <I/σ(I)> | 19.9 | 4.5 |
| Completeness [%] | 99.8 | 99.6 |
| Redundancy | 18.7 | 17.3 |
| CC(1/2) | 0.999 | 0.968 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 0.1 M LiCl, 0.1 MMES pH 6.0, 20% (m/v) PEG 6000 |
