6NU9
Crystal Structure of a Zinc-Binding Non-Structural Protein from the Hepatitis E Virus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E+ SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-10-09 |
Detector | DECTRIS PILATUS3 R 300K |
Wavelength(s) | 1.5418 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 64.399, 64.399, 158.901 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 52.624 - 1.761 |
R-factor | 0.2385 |
Rwork | 0.237 |
R-free | 0.26910 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | in-house model |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | PHENIX (1.14_3260) |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.624 | 1.820 |
High resolution limit [Å] | 1.761 | 1.760 |
Rmerge | 0.228 | 1.028 |
Rpim | 0.038 | 0.334 |
Number of reflections | 20137 | 1960 |
<I/σ(I)> | 13.4 | |
Completeness [%] | 99.9 | |
Redundancy | 33.7 | |
CC(1/2) | 0.997 | 0.339 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | 18% w/v PEG3350, 180 mM lithium nitrate, 10 mM nickel chloride |