6NU8
Structure of sucrose-6-phosphate hydrolase from Lactobacillus gasseri in complex with fructose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE W01B-MX2 |
| Synchrotron site | LNLS |
| Beamline | W01B-MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-17 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.4587 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 103.860, 103.860, 102.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.890 - 1.800 |
| R-factor | 0.13596 |
| Rwork | 0.134 |
| R-free | 0.16532 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.816 |
| Data reduction software | TRUNCATE |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.890 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.092 | 0.272 |
| Rmeas | 0.096 | 0.345 |
| Rpim | 0.029 | 0.209 |
| Number of reflections | 51784 | 2668 |
| <I/σ(I)> | 17 | 2.7 |
| Completeness [%] | 99.1 | 88.2 |
| Redundancy | 18 | 4.3 |
| CC(1/2) | 0.999 | 0.895 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 0.1 M buffer MMT pH 9.0; 25% (m/v) PEG 1500 |
