6NTY

2.1 A resolution structure of the Musashi-2 (Msi2) RNA recognition motif 1 (RRM1) domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	30.795, 57.370, 41.315
Unit cell angles	90.00, 101.32, 90.00

Refinement procedure

Resolution	33.092 - 2.100
R-factor	0.2096
Rwork	0.205
R-free	0.27970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5c8u
Data reduction software	XDS
Data scaling software	Aimless (0.6.3)
Phasing software	PHASER (2.8.2)
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.510	40.510	2.160
High resolution limit [Å]	2.100	8.910	2.100
Rmerge	0.112	0.073	0.801
Total number of observations	27686
Number of reflections	8271	121	675
<I/σ(I)>	6.5
Completeness [%]	99.4	98.6	99.3
Redundancy	3.3	3.2	3.2
CC(1/2)	0.993	0.996	0.559

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	2.4 M ammonium phosphate dibasic, 0.1 M Tris