6NTV

SFTSV L endonuclease domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2018-06-06
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97918
Spacegroup name	P 43 21 2
Unit cell lengths	82.732, 82.732, 229.089
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.090 - 2.400
R-factor	0.2021
Rwork	0.200
R-free	0.23800
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.855
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((1.13_2998))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.090	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.122	0.768
Rmeas	0.132	0.824
Rpim	0.047	0.294
Number of reflections	32031	2936
<I/σ(I)>	14.02	4.1
Completeness [%]	98.9	93.83
Redundancy	7.7	8
CC(1/2)	0.997	0.798

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	10% PEG 20K, 0.1 M Na Citrate pH 5