6NS1
Crystal structure of DIP-gamma IG1+IG2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2015-11-24 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97919 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 29.330, 43.440, 86.140 |
| Unit cell angles | 90.00, 90.46, 90.00 |
Refinement procedure
| Resolution | 43.069 - 1.850 |
| R-factor | 0.2059 |
| Rwork | 0.204 |
| R-free | 0.23460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5eo9 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.956 |
| Data reduction software | XDS (Oct 15, 2015) |
| Data scaling software | XDS (Oct 15, 2015) |
| Phasing software | PHASER (2.6.0) |
| Refinement software | PHENIX (dev_2932) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.900 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.146 | 0.716 |
| Rmeas | 0.158 | 0.833 |
| Number of reflections | 18742 | 1366 |
| <I/σ(I)> | 8.81 | 1.6 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 6.32 | 3.79 |
| CC(1/2) | 0.996 | 0.828 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 294 | 0.15 M ammonium sulfate, 0.1 M MES, pH 5.5, 25% (w/v) PEG 4000 |
