6NS1

Crystal structure of DIP-gamma IG1+IG2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	120
Detector technology	CCD
Collection date	2015-11-24
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97919
Spacegroup name	P 1 21 1
Unit cell lengths	29.330, 43.440, 86.140
Unit cell angles	90.00, 90.46, 90.00

Refinement procedure

Resolution	43.069 - 1.850
R-factor	0.2059
Rwork	0.204
R-free	0.23460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5eo9
RMSD bond length	0.008
RMSD bond angle	0.956
Data reduction software	XDS (Oct 15, 2015)
Data scaling software	XDS (Oct 15, 2015)
Phasing software	PHASER (2.6.0)
Refinement software	PHENIX (dev_2932)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.900
High resolution limit [Å]	1.850	1.850
Rmerge	0.146	0.716
Rmeas	0.158	0.833
Number of reflections	18742	1366
<I/σ(I)>	8.81	1.6
Completeness [%]	99.9	99.8
Redundancy	6.32	3.79
CC(1/2)	0.996	0.828

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	294	0.15 M ammonium sulfate, 0.1 M MES, pH 5.5, 25% (w/v) PEG 4000