6NRV
Cryo-EM reconstruction of CFA/I pili
EM specimen
Vitrification | Yes |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Detector | FEI FALCON II (4k x 4k) |
3D Reconstruction
Method | HELICAL |
Resolution (Å) | 4 |
Number of Particle | 117011 |
Software | EMAN2,SPIDER,Coot,PHENIX,EPU,CTFFIND,RosettaEM |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |