6NRR

Crystal structure of Dpr11 IG1 bound to DIP-gamma IG+IG2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	120
Detector technology	CCD
Collection date	2014-10-06
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.96638
Spacegroup name	P 43 21 2
Unit cell lengths	85.364, 85.364, 103.584
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.279 - 2.500
R-factor	0.21
Rwork	0.207
R-free	0.26070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5eo9
RMSD bond length	0.005
RMSD bond angle	0.699
Data reduction software	XDS (January 10, 2014)
Data scaling software	XDS (January 10, 2014)
Phasing software	PHASER (2.5.7)
Refinement software	PHENIX (dev_3374)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.650
High resolution limit [Å]	2.500	2.500
Rmerge	0.161	1.818
Rmeas	0.168	1.931
Number of reflections	13814	2138
<I/σ(I)>	16.48	1.17
Completeness [%]	99.7	98.1
Redundancy	13.26	8.61
CC(1/2)	0.998	0.577

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	294	0.1 M sodium citrate, pH 5.5, 2 M ammonium sulfate