6NRQ

Crystal structure of Dpr10 IG1 bound to DIP-alpha IG1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	120
Detector technology	PIXEL
Collection date	2018-02-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0332
Spacegroup name	P 1
Unit cell lengths	51.011, 53.551, 56.685
Unit cell angles	119.68, 103.77, 92.88

Refinement procedure

Resolution	48.551 - 1.800
R-factor	0.1759
Rwork	0.174
R-free	0.20540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5eo9
RMSD bond length	0.008
RMSD bond angle	0.938
Data reduction software	XDS (Nov 11, 2017)
Data scaling software	XDS (Nov 11, 2017)
Phasing software	PHASER (2.8.1)
Refinement software	PHENIX (dev_3112)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.910
High resolution limit [Å]	1.800	1.800
Rmerge	0.033	0.515
Rmeas	0.046	0.721
Number of reflections	40135	4014
<I/σ(I)>	11.77	1.31
Completeness [%]	86.8	53.8
Redundancy	1.82	1.67
CC(1/2)	0.999	0.670

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	294	1 M lithium chloride, 0.1 M HEPES, pH 7.0, 20% (w/v) PEG 6000