6NRO
Human parainfluenza virus type 3 fusion protein N-terminal heptad repeat domain+VIQKI
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-03-25 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.033 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.970, 52.250, 54.110 |
Unit cell angles | 90.00, 100.56, 90.00 |
Refinement procedure
Resolution | 39.293 - 1.750 |
R-factor | 0.2192 |
Rwork | 0.217 |
R-free | 0.24440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ztm |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.293 | 1.813 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmeas | 0.049 | 1.238 |
Rpim | 0.019 | 0.480 |
Number of reflections | 22158 | 2189 |
<I/σ(I)> | 18.26 | 1.45 |
Completeness [%] | 99.5 | 99.95 |
Redundancy | 6.6 | 6.5 |
CC(1/2) | 1.000 | 0.726 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 30 mM NaF, 30 mM NaBr, 30 mM NaI, 20% (v/v) PEG 500 MME, 10% (w/v) PEG 20000 in 100 mM imidazole/MES monohydrate buffer (pH 6.5)20% (v/v) PEG 500 MME, 10% (w/v) PEG 20000 in 100 mM imidazole/MES monohydrate buffer (pH 6.5) |