6NR7
Rerefinement of chicken vinculin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-06-07 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97955 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 56.020, 126.947, 351.870 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.960 - 3.000 |
| R-factor | 0.22382 |
| Rwork | 0.219 |
| R-free | 0.31466 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1st6 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.589 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.960 | 3.100 |
| High resolution limit [Å] | 2.990 | 2.990 |
| Rmerge | 0.092 | |
| Number of reflections | 25853 | 2481 |
| <I/σ(I)> | 17.1 | |
| Completeness [%] | 99.0 | |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 303 | ammonium sulphate, cacodylic acid, dtt, pH 6.5 |
