6NQZ

Flagellar protein FcpB from Leptospira interrogans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	109
Detector technology	IMAGE PLATE
Collection date	2012-04-28
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.54179
Spacegroup name	P 21 21 21
Unit cell lengths	60.735, 65.653, 134.396
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.010 - 2.580
R-factor	0.206
Rwork	0.204
R-free	0.25000
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.110
Data reduction software	TRUNCATE
Data scaling software	SCALA (3.3.20)
Phasing software	SHELXCD
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	67.232	37.151	2.720
High resolution limit [Å]	2.577	8.150	2.580
Rmerge		0.051	0.395
Rmeas	0.150	0.061	0.464
Rpim	0.078	0.033	0.241
Number of reflections	17479	634	2442
<I/σ(I)>	8.1	11.4	1.9
Completeness [%]	99.2	99.1	97.1
Redundancy	3.6	3.1	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	(NH4)I, PEG 3350, MES, Glycerol