6NQY

Flagellar protein FcpA from Leptospira biflexa / ab-centered monoclinic form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-11-23
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97858
Spacegroup name	C 1 2 1
Unit cell lengths	82.321, 99.586, 106.686
Unit cell angles	90.00, 91.95, 90.00

Refinement procedure

Resolution	40.290 - 2.500
R-factor	0.197
Rwork	0.196
R-free	0.22100
RMSD bond length	0.010
RMSD bond angle	1.030
Data reduction software	TRUNCATE
Data scaling software	Aimless (0.1.27)
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.120	45.120	2.600
High resolution limit [Å]	2.500	9.010	2.500
Rmerge	0.040	0.023	0.484
Rmeas	0.047	0.028	0.577
Rpim	0.025	0.015	0.313
Number of reflections	29300	648	3278
<I/σ(I)>	18.2
Completeness [%]	98.4	97.4	97.9
Redundancy	3.4	3.1	3.3
CC(1/2)	0.999	0.999	0.805

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG 400, TrisHCl