6NQX

Flagellar protein FcpA from Leptospira biflexa / primitive monoclinic form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-11-23
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97857
Spacegroup name	P 1 21 1
Unit cell lengths	85.475, 96.465, 121.138
Unit cell angles	90.00, 105.19, 90.00

Refinement procedure

Resolution	42.700 - 2.950
R-factor	0.198
Rwork	0.197
R-free	0.22200
RMSD bond length	0.010
RMSD bond angle	1.000
Data reduction software	TRUNCATE
Data scaling software	Aimless (0.1.27)
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.230	48.230	3.070
High resolution limit [Å]	2.950	10.640	2.950
Rmerge	0.050	0.016	0.651
Rmeas	0.059	0.020	0.768
Rpim	0.031	0.011	0.405
Number of reflections	39975	874	4485
<I/σ(I)>	17.2
Completeness [%]	99.5	96.8	99.7
Redundancy	3.4	3.2	3.5
CC(1/2)	0.999	0.999	0.842

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG 4000, TrisHCl