6NQW

Flagellar protein FcpA from Leptospira biflexa - hexagonal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-21
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97910
Spacegroup name	P 6 2 2
Unit cell lengths	132.360, 132.360, 67.360
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	58.080 - 1.900
R-factor	0.195
Rwork	0.194
R-free	0.21900
Structure solution method	AB INITIO PHASING
RMSD bond length	0.010
RMSD bond angle	0.860
Data reduction software	TRUNCATE
Data scaling software	Aimless (0.5.29)
Phasing software	Arcimboldo
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	67.360	67.360	1.940
High resolution limit [Å]	1.900	9.100	1.900
Rmerge	0.152	0.047	2.128
Rmeas	0.166	0.053	2.309
Rpim	0.066	0.025	0.880
Number of reflections	27914	320	1742
<I/σ(I)>	23.9
Completeness [%]	99.7	98.5	99.3
Redundancy	6.5	4.4	6.8
CC(1/2)	0.991	0.977	0.218

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.2	293	PEG 8000, Na/K phosphate, NaCl