6NQV
Crystal structure of fast switching M159T mutant of fluorescent protein Dronpa (Dronpa2), Y63(3-CH3Y)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL14-1 |
Synchrotron site | SSRL |
Beamline | BL14-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-05-23 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.195 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 79.988, 85.729, 143.006 |
Unit cell angles | 90.00, 94.91, 90.00 |
Refinement procedure
Resolution | 39.344 - 2.700 |
R-factor | 0.2567 |
Rwork | 0.255 |
R-free | 0.29530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4uts |
RMSD bond length | 0.009 |
RMSD bond angle | 1.236 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.13rc2_2986: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.344 | 2.770 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmeas | 0.200 | 1.380 |
Number of reflections | 51870 | 3833 |
<I/σ(I)> | 7.7 | 1.6 |
Completeness [%] | 97.4 | 98.4 |
Redundancy | 7.1 | 7.4 |
CC(1/2) | 0.997 | 0.842 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 293 | 0.1 M Tris-HCl at pH 7.4, 0.1 M MgCl2, 18% PEG 3350 |