6NQS
Crystal structure of fast switching M159T mutant of fluorescent protein Dronpa (Dronpa2)- Y63(3-OMeY)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL14-1 |
Synchrotron site | SSRL |
Beamline | BL14-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-04-22 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.195 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 82.396, 86.489, 144.082 |
Unit cell angles | 90.00, 94.96, 90.00 |
Refinement procedure
Resolution | 39.870 - 2.500 |
R-factor | 0.215 |
Rwork | 0.214 |
R-free | 0.25100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4uts |
RMSD bond length | 0.007 |
RMSD bond angle | 1.364 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.13RC2_2986: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.866 | 2.560 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmeas | 0.139 | 1.120 |
Number of reflections | 69116 | 5144 |
<I/σ(I)> | 10.7 | 2.1 |
Completeness [%] | 98.4 | 99.6 |
Redundancy | 7.7 | 8 |
CC(1/2) | 0.999 | 0.874 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 293 | 0.1 M Tris-HCl at pH 7.4, 0.1 M MgCl2, 16% PEG 3350 |