6NQN

Crystal structure of fast switching M159T mutant of fluorescent protein Dronpa (Dronpa2), Y63(3-BrY)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 1
Unit cell lengths	71.390, 79.490, 85.650
Unit cell angles	89.92, 92.40, 94.89

Refinement procedure

Resolution	37.610 - 2.100
R-factor	0.173
Rwork	0.172
R-free	0.20400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4uts
RMSD bond length	0.007
RMSD bond angle	1.192
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.13RC2_2986: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.610	2.150
High resolution limit [Å]	2.100	2.100
Rmeas	0.139	0.840
Number of reflections	106887	7389
<I/σ(I)>	9.2	1.8
Completeness [%]	97.7	91.6
Redundancy	6	4.3
CC(1/2)	0.996	0.691

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Tris-HCl at pH 7.4, 0.1 M MgCl2, 15% PEG 3350