6NQM

Crystal structure of Human LSD1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-02-07
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	P 61 2 2
Unit cell lengths	189.401, 189.401, 107.104
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.890 - 2.900
R-factor	0.2141
Rwork	0.212
R-free	0.24450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2hko
RMSD bond length	0.004
RMSD bond angle	0.709
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.890	3.080
High resolution limit [Å]	2.900	2.900
Rmeas	0.244
Rpim	0.054
Number of reflections	25582
<I/σ(I)>	11.9	4.5
Completeness [%]	99.9	100
Redundancy	38
CC(1/2)	0.999	0.551

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	296	PEG 3350 8%, 0.1 M Ammonium citrate pH6.5